All about Donkey Milk

How much do you use? We started slow, but worked up to a cup a day, 1/2 am, 1/2 pm (she’s 6, 50 lbs).  Mixed with a bit of pure Grade B maple syrup.  Contact Dr. Hinkle at for a specific plan for your child.  She’s the one who told us donkey milk is just as good as camel milk, and some kids tolerate one and not the other.  But be prepared, she can be…blunt! For an excellent study on Autistic kids and Donkey Milk, scroll way way down to Camel Milk/ Donkey Milk study More information on donkey milk, “like” Donkey Milk USA and join the facebook page “Healing with Donkey Milk” Below is my ever updating list of articles, documents, and research on Donkey Milk and it’s benefits.  Bookmark this page and check back often!  It’s exciting stuff. Anti Inflammatory & Immunological Properties of Donkey Milk “From an immunological point of view, donkey’s milk is able to induce release of inflammatory and anti-inflammatory cytokines from normal human peripheral blood lymphomononuclear cells, thus maintaining a condition of immune homeostasis.” Donkey Milk Analysis & Historical Background Donkey Milk Studies & Literature Donkey Milk vs. Camel Milk Technical Journal Donkey Milk for babies & children allergic to cow milk & soy Study on the benefits of donkey milk….anti-tumor, immunological, for preventing atherosclerosis (hardening of arteries), and probiotic properties Donkey Milk for skin health / beauty and milk properties Donkey Milk used for Whooping Cough, Osteoporosis, and Calcium absorption and other studies from Eurolactis A study on donkey milk in Italy….the rats on cows milk fattened up, the rats on donkey milk were super energized. “And their mitochondria – the tiny batteries that power cells – were super-charged, turning food into energy at a fast pace” NIH (National Institute of Health) Research Studies on Donkey Milk:

  • Anti-Inflammatory and Anti-allergenic Properties …”in infants with intolerance to cow’s milk, donkey’s milk represents a good alternative due to its chemical characteristics similar to those of human milk….From an immunological point of view, donkey’s milk is able to induce release of inflammatory and anti-inflammatory cytokines from normal human peripheral blood lymphomononuclear cells, thus maintaining a condition of immune homeostasis….in these milks the presence of their own microbiota may normalize the human intestinal microbiota with a cascade of protective effects at intestinal mucosal sites, even including triggering of intestinal T regulatory cells.”
  • Hypoallergenic properties of donkey’s milk: a preliminary study. “Cow’s milk protein allergy (CMPA) is an abnormal immunological response to cow milk proteins, which results in IgE-mediated reactions. The therapeutic strategy to respond to CMPA envisages the total elimination of milk or the administration of cow’s milk substitutes. For this reason the use of milk from other mammalian species was tested. Among them, donkey’s milk proved to be the best alternative in feeding infants affected by CMPA, since its chemical composition is comparable to human milk.”
  • Efficacy of donkey’s milk in treating highly problematic cow’s milk allergic children: an in vivo and in vitro study. “Successful therapy in cow’s milk protein allergy rests on completely eliminating cow’s milk proteins from the child’s diet: it is thus necessary to provide a replacement food. This prospective study investigated tolerance of donkey’s milk in a population of 46 selected children with cow’s milk protein allergy, for whom it was not possible to use any cow’s milk substitute. Thirty-eight children (82.6%) liked and tolerated donkey’s milk at the challenge and for the entire duration of follow-up. Catch-up growth was observed in all subjects with growth deficit during cow’s milk proteins challenge. The degree of cross-reactivity of immunoglobulin E (IgE) with donkey’s milk proteins was very weak and aspecific. Donkey’s milk was found to be a valid alternative to both IgE-mediated and non-IgE-mediated cow’s milk proteins allergy, including in terms of palatability and weight-height gain.”
  • Tolerability of donkey’s milk in 92 highly-problematic cow’s milk allergic children. “STUDY DESIGN: A prospective study was conducted on 92 children with CMA, diagnosed through a CM elimination diet, followed by double-blind, placebo-controlled food challenge (DBPCFC) unless contraindicated. Maternal milk was unavailable and current CM substitutes could not be used. Moreover, 89 percent were affected by multiple FA, and subjected to very restricted diets. Within 3 months after the last CM challenge, DBPCFC for DM was performed. CM or DM skin prick test and sIgE determination preceded the CM or DM challenge, respectively. Native electrophoresis and immunoblotting were used to identify CM and DM cross-reactive proteins. Z-scores of weight and length/stature for age were calculated at DM food challenge (T0) and during DM assumption.
    • RESULTS: 83 children (90.2 percent) liked and tolerated DM, at challenge and during follow-up, with increased Z-score for weight and length/stature and improved nutritional parameters. Bovine beta-lactoglobulin was identified as the cross-reacting protein among the DM allergic patients.
    • CONCLUSIONS: DM was found to be a valid alternative foodstuff, in terms of clinical tolerability, palatability and nutritional adequacy, in subjects with CMA who were highly problematic from the feeding standpoint.”
  • Hypoallergenic properties of donkey’s milk: a preliminary study. “Cow’s milk protein allergy (CMPA) is an abnormal immunological response to cow milk proteins, which results in IgE-mediated reactions. The therapeutic strategy to respond to CMPA envisages the total elimination of milk or the administration of cow’s milk substitutes. For this reason the use of milk from other mammalian species was tested. Among them, donkey’s milk proved to be the best alternative in feeding infants affected by CMPA, since its chemical composition is comparable to human milk. In this work an in vitrostudy was performed in order to analyze the IgE reactivity to milk protein allergens from cow, donkey and goat. In particular, immunoblotting experiments using sera from milk-allergic and non-allergic adult volunteers were conducted with the aim of verifying the hypoallergenic property of donkey’s milk. This study provided a preliminary evidence of the hypoallergenicity of donkey’s milk when compared to bovine and goat milk” (Vincenzetti S, et al. Vet Ital. 2014 Apr-Jun.)
  • Donkey’s milk detailed lipid composition “Donkey’s milk (DM) has recently aroused scientific interest, above all among paediatric allergologists. A deeper knowledge of both proteins and fats in donkey’s milk is necessary to evaluate the immunological, physiological and nutritional properties. By using the most refined techniques for fatty acids analysis, the paper offers a detailed comparative analysis of the lipid fractions of DM as well as of human and cow milk, also indicating the distribution of fatty-acid moieties among sn-1/3 and sn-2 positions of the glycerol backbone. In DM the position of fatty acids on glycerol backbone, above all of long chain saturated fatty acids, is very similar to that of human milk: this fact, in conjunction with the relatively high contents of medium-chain triglycerides, makes the lipids in DM, through quantitatively reduced, highly bioavailable. The high PUFA n-3 content of donkey’s milk, and especially its low n-6/n-3 ratio, acquires particular interest in subjects affected by cow’s milk protein allergy. Whole DM might also constitute the basis for formulas suitable for subjects in the first year of life..” (Gastaldi D, et al. Front Biosci (Elite Ed). 2010.)
  • Profile of nucleosides and nucleotides in donkey’s milk “Nucleotides play a crucial role to cellular functions; they can be obtained from the diet or through the nucleotide salvage pathway, however, in particular situations (occurring mainly in newborns) the metabolic demand of nucleotides exceeds the capacity of their synthesis. These molecules, are receiving attention from a nutraceutical point of view because of their potential direct role in regulating metabolism and infant body condition. Donkey’s milk may be considered a good replacer for cow’s milk in feeding children with severe Ig-E mediated cow’s milk protein allergy, due to its high similarity with human milk. In this study, the presence of cytidine, uridine, CMP, UMP, guanosine, and adenosine, involved in numerous biochemical and physiological activities, were detected for the first time through a RP-HPLC method.”. (Vincenzetti S, et al. Nucleosides Nucleotides Nucleic Acids. 2014)
  • Identification of Equine Lactadherin-derived Peptides That Inhibit Rotavirus Infection via Integrin Receptor Competition “Human rotavirus is the leading cause of severe gastroenteritis in infants and children under the age of 5 years in both developed and developing countries. Human lactadherin, a milk fat globule membrane glycoprotein, inhibits human rotavirus infection in vitro, whereas bovine lactadherin is not active. Moreover, it protects breastfed infants against symptomatic rotavirus infections. To explore the potential antiviral activity of lactadherin sourced by equines, we undertook a proteomic analysis of milk fat globule membrane proteins from donkey milk and elucidated its amino acid sequence. Alignment of the human, bovine, and donkey lactadherin sequences revealed the presence of an Asp-Gly-Glu (DGE) α2β1 integrin-binding motif in the N-terminal domain of donkey sequence only. Because integrin α2β1 plays a critical role during early steps of rotavirus host cell adhesion, we tested a minilibrary of donkey lactadherin-derived peptides containing DGE sequence for anti-rotavirus activity. A 20-amino acid peptide containing both DGE and RGD motifs (named pDGE-RGD) showed the greatest activity, and its mechanism of antiviral action was characterized; pDGE-RGD binds to integrin α2β1 by means of the DGE motif and inhibits rotavirus attachment to the cell surface. These findings suggest the potential anti-rotavirus activity of equine lactadherin and support the feasibility of developing an anti-rotavirus peptide that acts by hindering virus-receptor binding.”. (Civra A, et al. J Biol Chem. 2015.)
  • Donkey Milk for Manufacture of Novel Functional Fermented Beverages “The aim of this work was to investigate on the functional features of a donkey milk probiotic berevage as a novel food. Particularly, it was to study the decrease of lactose content and the antioxidant activity of standard yogurt (YC) and probiotic yogurt (YP; Lactobacillus acidophilus, Lactobacillus casei) from donkey milk during the storage up to 30 d at 4 ºC. The evolution of lactose content using enzymatic-spectrophotometric kits was analyzed. Antioxidant activity of yogurt was measured using 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power (FRAP), and thiol assays. Parallel consumer sensory studies were carried out as consumer test in order to gain information about the impact of these novel fermented beverages on sensory perceptions. The statistical analysis has shown significant effect of studied factors. The results showed that the lactose content gradually decreased during storage in both yogurts, reaching values of 2.36% and 2.10% in YC and YP, respectively, at 30 d (P < 0.05). During storage of both yogurt types, the antioxidant activity increased, but YP showed a higher antioxidant activity than YC. The results suggest that the antioxidant activity of yogurt samples was affected by cultures of lactic acid bacteria (LAB). We conclude that the fermented donkey milk could be configured as health and nutraceutical food, which aims to meet nutritional requirements of certain consumers groups with lactose or cow milk protein intolerance.”. (Perna A, et al. J Food Sci. 2015.)  (***NOTE:  I would avoid making yogurt or kefir from Donkey milk (or any milk) if you have issues with Strep strains / PANDAS.  See Post about Strep in Yogurt and Kefir and Probiotics!)

Anti-proliferative and anti-tumour effect of active components in donkey milk on A549 human lung cancer cells:  “The anti-proliferative and anti-tumour effects of donkey milk on A549 human lung cancer cells were investigated in vitro, and its effects on cell cycle progression, apoptosis and cytokine production were examined. Donkey milk active fractions reduced the viability of A549 cells in dose-dependent and time-dependent manners. Fraction-IV, a fraction of whey protein with a molecular mass >10 kDa, was the most effective fraction (P < 0.05) in inducing an accumulation of A549 cells in the G0/G1 and G2/M phases, indicating a potent cytotoxicity and causing cell death by apoptosis. The anti-proliferative activity of conditioned medium, prepared with fraction-IV-stimulated murine splenocytes, was significantly higher than that of fraction-IV alone (P < 0.05). Moreover, it could increase the secretion of Interleukin-2 (IL-2), Interferon-γ (IFN-γ), Interleukin-6 (IL-6), tumour necrosis factor α (TNF-α) and Interleukin 1β (IL-1β). The active components of donkey milk not only could directly suppress tumour proliferation in vitro, but may also indirectly kill tumours through activation of lymphocytes and macrophages. A high content of lysozyme in fraction-IV may contribute to its anti-tumour activity.” Study by Xueying Mao1, Junnan Gu1, Yan Sun, Shiping Xu, Xiaoying Zhang, Haiying Yang, Fazheng Ren

Pope drank donkey milk as a baby Donkey milk and it`s benefits by Johannes Visagie:  Donkey Milk is…”A serum protein content similar to breast milk with a casein content significantly lower than cows’ milk; this promotes improved digestion and assimilation, reducing the allergenic potential A balanced relationship between the various essential fatty acids, with a predominance of those promoting anti-inflammatory and vascular protection effects; the easily digestible bio-available fat content makes donkey milk an optimum source of energy, particularly in demanding situations such as the practice of combat sports when it is essential to maintain a constant metabolic energy supply Enzymes and immuno-protective substances (lysine, lactoferrin, lactadherin) which boost the immune system and reduce the risk of intestinal infection…..” Donkey Milk for European Babies & Elderly Chinese Story of Donkey Milk for Whooping Cough in Africa (and other “crazy” remedies) Sciences Blog Donkey Milk as Immuno Modulator  Research articles via Eurolactis

Where do you get donkey milk? For more info and updated resources for Donkey Milk, join the FB Group Healing with Donkey Milk.  You can also search the “Files” section there for more updated articles and research. And if you speak French, or just want to see how the French milk their donkeys…here you go. —————————————— This blog is written by an anonymous mom. PANDAS disease is ugly. It’s practically unheard of, but it’s not rare. 1 in 200 kids has it. That’s right up there with childhood diabetes…but my pediatrician had never heard of it. This leads me to the question, how many kids out there are misdiagnosed, drugged for “behavioral issues”, OCD, schizophrenia, ADD, Autism, ADHD, Anorexic, or taken from their “bad” parents by well meaning doctors, teachers, and lawyers who don’t know about PANDAS. How many parents are searching for answers every night, in silent agony over losing their child, and have no one to talk to, no one who understands. This blog is for those parents. Those doctors. Those teachers. But mostly, it’s for those children. The ones with the fear in their eyes and the haunted look. The chapped and bloodied hands from washing incessantly. The lost childhood. Lord, multiply this, share it with those who need it most. I don’t have time, or resources, or knowledge to get it into their hands. If it will bless them, let this reach them. In Jesus Name I pray for each hurting child. Amen. Disclaimer: This blog exists for informational purposes only. Posts should NOT be regarded as medical advice. No author or commentator shall be liable for advice given. It is every parent’s responsibility to determine whether camel milk, donkey milk, specific supplements, the ACC protocol or other biomedical interventions are appropriate for their child. Furthermore, dosing for vitamins and supplements needs to be tailored to an individual’s needs with the help of a professional. Thank you for reading the blog, please share it with those in need of hope!


4 responses to “All about Donkey Milk

  1. How does donkey milk compare to goats milk? I only see comparisons to cows milk, but goat is far superior. Can you show me any comparisons to goat milk?

  2. Pingback: Daily Log: Lyme Disease ABX Protocol and PANDAS disease | Buttercup Farm·

  3. Pingback: Bee Pollen for PANDAS? Mom Fail. | Buttercup Farm·

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